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Fig. 6 | Molecular Brain

Fig. 6

From: Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults

Fig. 6

Absence of Arl13b-positive astrocytic primary cilia but robust Arl13b expression in tissues proximal to injured sites. a-b Increased Arl13b expression proximal to an injury site (b) compared to a non-injury site (A). Scale: 5 μm. Note that Arl13b-positive primary cilia were detected in non-injured tissues (a), but undetectable in injured tissues (b). c The percentage of ciliated cells near injury sites decreased dramatically compared to control tissues (left). ***, p < 0.001, unpaired student’s t-test. Injured tissues: total cell number: 665, ciliated cell N: 13; non-injured tissues: total cell number: 265, ciliated cell number: 47. Injured tissues had significantly increased percentage of Arl13b-positive cells compared to that of non-injury tissues (right). ***, p < 0.001, unpaired Student’s t-test, N = 3. Injury site cell N = 806; non-injury site cell number 600. Two Arl13b images from injured and non-injured sites are shown in the left. Non-injury sites were from the opposite hemisphere of the injury site of same mouse. d Increased Arl13b expression near injured sites in the cortex of C57BL/6 mice. GFAP (cyan) and Arl13b (red) were stained with their antibodies, respectively. Left, non-injured site; right, injured site. e Increased Arl13b expression near injured sites in Arl mice. Red: Arl13b-mCherry; Cyan: immunofluorescence staining using anti-GFAP antibody. f Distinct Arl13b expression density in injured and non-injured tissues. Arl13b had the highest expression near to the injury site. N = 5. g GFAP expression was strongly increased near to injured tissues compared to non-injured tissues. h Correlation of relative expression density of GFAP and Arl13b over a range of 500 μm distance from the injured site. ***, p < 0.001 by correlation analysis

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