Fig. 1From: Optical monitoring of glutamate release at multiple synapses in situ detects changes following LTP inductionMonitoring glutamate release from multiple axons ex vivo in hippocampal slices labelled with iGluSnFR through viral transduction in vivo. a A diagram depicting viral ICV injections in neonates (P0-P2) followed by AAV transduction (3–4 weeks), dissection of hippocampi, and acute slice preparation for two-photon excitation imaging coupled with electrophysiology (Schaffer collateral stimulation and fEPSP recording in S. radiatum). b Experimental arrangement as seen in the microscope (DIC channel); stimulating and recording electrodes are seen; dotted rectangle, ROI for imaging. c Image, axon fragment in S. radiatum (ROI as in B) as seen in the green channel (AAV9.hSynap.iGluSnFR.WPRE.SV40 fluorescence; 50-frame average). Upper trace, fEPSP response to afferent stimuli (five at 20 Hz, one-trial example); lower trace, the corresponding ROI-averaged ΔF/F0 signal time course (one-trial example). d Arrangement as in (b) but for the ‘slow-decay’ sensor variant AAV2/1.hSyn.SF.iGluSnFR.A184S (green channel shown). e Experiment as in (c) but for AAV2/1.hSyn.SF.iGluSnFR.A184S; notation as in (c)Back to article page