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Fig. 5 | Molecular Brain

Fig. 5

From: Optical monitoring of glutamate release at multiple synapses in situ detects changes following LTP induction

Fig. 5

LTP induction at CA3-CA1 synapses boosts optical glutamate signal in the S. radiatum neuropil. a Image, axon fragment in S. radiatum showing the area with multiple axonal boutons (dotted rectangle, iGluSnFR.WPRE.SV40 channel) for the analysis of average iGluSnFR ΔF/F0 signal (right traces), as shown before (pre), ~ 30 min after (red), and 90 min after HFS. One-slice example; traces, singletrial examples; arrows and dotted lines, afferent stimulus timestamps. Averaging interval for calculating {ΔF/F0} values is shown. b ROI-average iGluSnFR {ΔF/F0} values in baseline conditions (pre), and at 30 min and 90 min after LTP induction, as indicated. Connected dots, individual slice data; bars, average values (n = 7). *p < 0.04; ***p < 0.005. c Average iGluSnFR ΔF/F0 signal traces (line ± shaded area, mean ± SEM, n = 7) normalised to their {ΔF/F0} value in baseline conditions, in each individual preparation, and rescaled to illustrate the ‘average ΔF/F0 traces’ across preparations (ΔF/F*). d Experiment as in (a) but following the blockade of glutamate transporters with 50 μM TBOA, at 90 min after LTP induction. fEPSP and iGluSnFR traces illustrate single trials recorded at different time points after TBOA application onset, as indicated; one-slice example, notations as in (a). Note that no ΔF/F0 signal (red) may reflect saturation of the baseline fluorescence F0

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