Fig. 1
From: Human iPS cell-derived mural cells as an in vitro model of hereditary cerebral small vessel disease
Differentiation of iPSC derived MCs. iPSCs were treated by GSK3β inhibitor, then StemPro34 and VEGF165, to obtain Flk-1 positive/VE-Cadherin negative mural cells (MCs, a). Differentiated mural cells were immature at the time of the sorting (b) and further cultured for maturation. MCs expressed VSMC marker αSMA, SM22 and calponin (c-e). Endothelial cells (ECs) can be also isolated as Flk-1 positive/VE-Cadherin positive cells. MCs differentiated by modified protocol changed their morphology depending on the culture condition (g and i, phenotypic transition), but not MCs differentiated by original protocol (f and h). Control MC (TIG107) differentiated in αMEM supplemented with 5%FBS and PDGF-BB showed markedly low PDGFRβ, irrespective of the coating (j, lane F and H). Bars represent 20 μm in (c-e), 150 μm in (b, f-i)