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Fig. 2 | Molecular Brain

Fig. 2

From: The origin and development of subcortical U-fibers in gyrencephalic ferrets

Fig. 2

Gallyas myelin staining and Turnbull blue iron staining using sections of adult ferrets. a, b Gallyas myelin staining. a Lower magnification images of the cerebral cortex. b Higher magnification images of the boxed area in (a). Note that the U-fiber region was densely labeled (square bracket). c Quantification of staining intensities of Gallyas myelin staining. Staining intensities of U-fiber regions were significantly higher than those in the deep white matter. Bars present mean ± SD. *p < 0.05; Welch’s t-test; n = 3 animals. d, e Turnbull blue iron staining. d Lower magnification images of the cerebral cortex. e Higher magnification images of the boxed area in (d). Note that the U-fiber region was densely labeled (square bracket). f Quantification of staining intensities of Turnbull blue iron staining. Staining intensities of U-fiber regions were significantly higher than those in the deep white matter. Bars present mean ± SD. *p < 0.05; Welch’s t-test; n = 3 animals. GM, gray matter; WM, white matter. Scale bars = 500 μm (a, d) and 300 μm (b, e)

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