Fig. 3

Distribution patterns of oligodendrocytes, astrocytes and microglia around the white matter. In situ hybridization for PLP (a) and immunohistochemistry for GFAP (b) and Iba-1 (c) were performed using brain sections of adult ferrets. Lower magnification Hoechst images are shown on the left. In situ hybridization, immunostaining and Hoechst images corresponding to the boxed areas in the lower magnification images are shown on the right. Note that oligodendrocytes, astrocytes and microglia were similarly distributed in the U-fiber region and the deep white matter. GM, gray matter; WM, white matter. Scale bars = 2 mm (left) and 200 μm (right)