Fig. 2
From: Role of lateral amygdala calstabin2 in regulation of fear memory
Lateral amygdala (LA) calstabin2 knockdown (KD) impairs long-term cued fear memory. a Representative AAV-infected neurons (green) in the LA. Scale bar: 200 μm. b Western blot showing calstabin2 protein levels in the LA of naive mice, shRNA-NC infected mice, and calstabin2 KD mice. Left: representative Western blot. Right: calstabin2 expression histograms. The y-axis indicates the calstabin2 protein expression level for each condition relative to naive control. The calstabin2 protein level for each condition was normalized to the BMP level. N = 3 for each group. Statistics, One-way ANOVA and Bonferroni post-hoc test. ***p < 0.001 compared with naive and shRNA-NC groups. c LA calstabin2 KD did not affect freezing response during the habituation (day 1: F1, 20 = 1.125, p = 0.301, ANOVA with repeated measures) and conditioning phases (day 2: F1, 20 = 0.378, p = 0.545, ANOVA with repeated measures). During the test phase, calstabin2 KD mice froze less often to conditioned stimulus than shRNA-NC infected ones did (day 3: ***p < 0.001, independent student’s t test)