Fig. 7

Effects of miRNA-326 on cell apoptosis under OGD. (a) The cell apoptosis rate did not change immediately at 0 h after OGD (ⁿP > 0.05). Apoptosis was significantly increased in PC12 cells transfected with miR-326 mimics and was significantly decreased in PC12 cells transfected with miR-326 inhibitor at 2 h, 6 h and 12 h after OGD (^**P < 0.01; ^***P < 0.005; ^****P < 0.001). In PC12 cells cotransfected with shDOR and miR-326 inhibitor, the cell apoptosis rate that resulted from the miR-326 inhibitor was partially rescued (^**P < 0.01; ^***P < 0.005). (b) Overexpression of miR-326 increased the expression levels of Caspase-3 and Bax and decreased Bcl-2 at 2 h, 6 h and 12 h after OGD but not at 0 h (ⁿP > 0.05; ^**P < 0.01; ^***P < 0.005; ^****P < 0.001). Inhibition of miR-326 had opposite effects, which were rescued by cotransfection of miR-326 and DOR inhibitors (^*P < 0.05; ^**P < 0.01; ^***P < 0.005; ^****P < 0.001)