Fig. 2

Immunofluorescence localization of AQP4 in retina. Confocal image showing AQP4 (in red), the Müller cell marker glutamine synthetase (GS; in green) and the endothelial marker lectin (in blue). AQP4 is concentrated in the perivascular (arrows) and in subvitreal membrane domains (arrowheads) in the WT (a and d) and β1-syn KO (b and e) animals. Perivascular AQP4 staining is strongly reduced in αβ1-syn KO mice (c and f). In both the knockouts, AQP4 labeling is increased along the entire length of Müller cell and around the inner nuclear layer. Nuclear staining is shown in white. GCL-ganglion cell layer; IPL-inner plexiform layer; INL-inner nuclear layer; OPL-outer plexiform layer; ONL-outer nuclear layer. Scale bars = 20 μm