Fig. 4

Quantitative analysis of the AQP4 immunogold labeling in different subregions of retina. a Schematic figure comparing the AQP4 distribution at the perivascular endfeet of the WT Müller cells in different layers i.e. outer plexiform layer (OPL), inner plexiform layer (IPL), ganglion cell layer (GCL) and the subvitreal endfeet (Sub). The AQP4 immunogold labeling (n = 4 mice) in Sub was significantly lower than GCL, IPL and OPL. b Quantitative analysis of AQP4 immunogold labeling in different retinal layers of WT, β1-syn KO and αβ1-syn KO mice. In mice lacking β1-syn, there was a significant reduction in the mean linear density of gold particles when compared with WT controls in all layers. In the double knockout mice, there was a near complete loss of perivascular labeling of AQP4 when compared with WT controls in all the layers (n = 4 for each genotype). Statistics: one-way ANOVA and post hoc Scheffé test. Data shown as mean ± SEM. * p < .05; ** p < .01; *** p < .001