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Fig. 5 | Molecular Brain

Fig. 5

From: Functional specialization of retinal Müller cell endfeet depends on an interplay between two syntrophin isoforms

Fig. 5

AQP4 localization requires the presence of both α1- and β1-syn in subvitreal domain. AQP4 (in red) is highly concentrated in the subvitreal membrane domains (arrows in a) in the WT animals. Deletion of either α1-syn (arrows in b) or β1-syn (arrows in c) alone does not affect AQP4 localization. Concomitant deletion of both α1- and β1-syn results in near complete loss of AQP4 (arrows in d). Nuclear staining is shown in white and Müller cell marker, glutamine synthetase (GS), is shown in green. Scale bar = 20 μm. Representative high resolution immunogold electron micrographs showing AQP4 localization in subvitreal domain. Concomitant deletion of both α1- and β1-syn results in near complete loss of AQP4 in subvitreal domain (e to h). Quantitative immunogold analysis shows a near complete loss of AQP4 in subvitreal domain (i; n = 4 for each group). Statistics: one-way ANOVA and post hoc Scheffé test. The arrowheads point to the endfoot domain facing the vitreous. Vit-vitreous body; * = basement membrane. Data shown as mean ± SEM. Scale bar = 200 nm. *** p < .001

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