Fig. 6

α1-syn protein level is increased in retinae of β1-syn KO mice. a Immunoblot showing α1-syn expression in total retinal protein lysates from WT and β1-syn KO retinae. Statistical analysis of α1-syn expression in WT and β1-syn KO shows that α1-syn protein level is significantly increased in β1-syn KO retina compared to WT (b; n = 5 for each group). Statistics: independent samples t-test. Densitometric values are expressed as percentage of average WT values ± SD. * p < .05. GAPDH was used as the loading control. Quantitative immunogold analysis showing α1-syn is increased in β1-syn KO retina, both in perivascular domain (c; n = 4 for each group) and in subvitreal domain (d; n = 4 for each group). Statistics: one-way ANOVA and post hoc Scheffé test. Data shown as mean ± SEM. *** p < .001