Fig. 4

BDNF intracellular production and release affects all synaptic components. In (a) representative voltage clamp recordings of spontaneous activity in tGFP (green trace) and tBDNF (red trace; 24 h) cultures. In (b) plots reporting PSCs amplitude (left) and frequency (right), significantly (P < 0.001, two-way ANOVA) increased by BDNF expression (24 h and 48 h). In (c) plots reporting EPSCs (left) and IPSCs (right) frequency, significantly (P < 0.001, two-way ANOVA) increased by intracellular BDNF (24 h and 48 h) compared to Control sister cultures. In (d) plots reporting EPSCs (left) and IPSCs (right) amplitude significantly (P < 0.001, two-way ANOVA) increased by intracellular BDNF (24 h and 48 h) compared to Control sister cultures. In (e) and (f) EPSC and IPSC frequency and amplitude values are depicted recorded from ne-tBDNF. In respect of Control (tGFP) only EPSP frequency values were significantly affected (P < 0.01 24 h, P < 0.05 48 h, two-way ANOVA)