Fig. 5

Colocalization of TrkB-full length receptor and VGAT. In (a) confocal images showing hippocampal neurons (Control culture) (anti-TrkB, in green; anti-VGAT, in red; nuclei are visualized by Hoechst in blue). Merge of the TrkB and VGAT channels or merge of the three channels are shown in dual o three-colour. The black and white image shows the colocalized TrkB and VGAT pixels. Note the presence of TrkB receptors at inhibitory synapses in the soma and the proximal domains of the apical and basal dendrites. Calibration bar: 10 μm. In (b) schematic summary of the proposed actions of BDNF when locally released at glutamatergic or GABAergic synapses