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Fig. 7 | Molecular Brain

Fig. 7

From: Ascending noradrenergic excitation from the locus coeruleus to the anterior cingulate cortex

Fig. 7

Activation of LC-ACC pathway facilitates the itch and pain sensations. a Diagram of stereotaxic virus injection into the LC of DBH-Cre mice and optic cannula implantation in the ACC. b Figures showing that optostimulation of the LC-ACC projecting NAergic fibers (Green) through the optic cannula induced strong Fos expression (Red) within the ACC. c Experimental schema of behaviour test. d The bouts and duration of scratching are increased after in vivo optostimulation in mice with ChR2 infection. * P < 0.05, **, P < 0.01, ***, P < 0.001, in comparison with eYFP-20 Hz group (n = 7 mice in each group). Two-Way ANOVA. e The number and duration of wiping are increased after in vivo optostimulation. * P < 0.05, **, P < 0.01,***, P < 0.001, in comparison with eYFP-20 Hz group. Two-Way ANOVA. f The paw withdrawal thresholds are decreased in both left and right hind paws after optostimulation (left hindpaw: eYFP-20 Hz: pre, 0.96 ± 0.09 g, post, 0.79 ± 0.09 g, P > 0.05; ChR2–5 Hz: pre, 0.90 ± 0.10 g, post, 0.55 ± 0.08 g, P < 0.05; ChR2–20 Hz: pre, 0.88 ± 0.12 g, post, 0.32 ± 0.09 g, P < 0.01; right hindpaw: eYFP-20 Hz: pre, 0.90 ± 0.09 g, post, 0.73 ± 0.14 g, P > 0.05; ChR2–5 Hz: pre, 0.80 ± 0.16 g, post, 0.55 ± 0.17, P > 0.05; ChR2–20 Hz: pre, 0.76 ± 0.12 g; post, 0.42 ± 0.10 g, P < 0.05. n = 6 mice in each group). Paired t-test

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