Fig. 1From: A mutant vesicular stomatitis virus with reduced cytotoxicity and enhanced anterograde trans-synaptic efficiencyConstruction of recombinant VSV with mutant N gene. To construct the recombinant mutant VSV vector, N gene with the seventh amino acid mutation (R to A) was amplified and inserted into the pVSV-EGFP digested by restriction enzyme MscI and BSTZ17IBack to article page