Fig. 4

Anterograde trans-synaptic labeling with Mutant-VSV after injection into the VTA. a Schematic of recombinant VSV anterograde trans-synaptic labeling. b The brain slices of injection site at indicated time points (3 DPI and 5 DPI) were imaged. c Anterograde trans-synaptic labeling with Mutant-VSV at indicated time points. Mutant VSV can effectively infect downstream brain area of VTA, and more brain regions were labeled over time, but less brain regions were labeled by Mutant-VSV than by WT-VSV at 5 DPI, which showed that Mutant-VSV had delayed anterograde trans-synaptic ability. The EGFP signal was imaged by using the Olympus VS120 Slide Scanner microscope. Scale bars =500 μm for B and C. The specific name of the brain areas labeled were shown as follows: AON: Anterior olfactory nucleus; TTd: Taenia tecta, dorsal part; TTv: Taenia tecta, ventral part; LS: Lateral septal nucleus; ACB: Nucleus accumbens; NDB: Diagonal band nucleus; OT: Olfactory tubercle; CP: Caudoputamen; SS: Somatosensory areas; MPN: Medial preoptic nucleus; BST: Bed nuclei of the stria terminalis; GPe: Globus pallidus, external segment; SI: Substantia innominata; FS: Fundus of striatum; MA: Magnocellular nucleus; AAA: Anterior amygdalar area; Habenula: Habenula; AHN: Anterior hypothalamic nucleus; CEA: Central amygdalar nucleus; BMAa: Basomedial amygdalar nucleus, anterior part; AI: Agranular insular area; PH: Posterior hypothalamic nucleus; DMH: Dorsomedial nucleus of the hypothalamus; PF: Parafascicular nucleus; RSP: Retrosplenial area; TEa: Temporal association areas; PIR: Piriform area; CA3: Ammon’s horn Field CA3; DR: Dorsal nucleus raphe; PAG: Periaqueductal gray; CS: Superior central nucleus raphe