Fig. 1

Intratibia inoculation of Walker 256 carcinoma cells caused progressive hyperalgesia and bone destruction. a. Mechanical hypersensitivity arose from 6 days after modeling and lasted to the end of behavioral test, while the mechanical threshold of sham-operated rats kept stable. The data are presented as the mean ± SD, **P < 0.01, ***P < 0.001 compared with sham-operated rats at each timepoint; n = 6, per group. PWMT, paw withdrawal mechanical threshold; BL, baseline; sham, sham-operated group; BCP, bone cancer pain group. b. X-ray examination of left hind paw showed discontinuity and destruction of cortical bone (marked by the arrow) and swelling of soft tissue in proximal tibia of bone cancer pain rats. c. Microscopic examination of tibia slices, by HE staining, showed severe destruction and disarrangement of bone trabecula in bone cancer pain rats, scale bar = 500 μm