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Fig. 2 | Molecular Brain

Fig. 2

From: Transcriptomic analysis of long noncoding RNAs and mRNAs expression profiles in the spinal cord of bone cancer pain rats

Fig. 2

Tumor inoculation caused significant changes in mRNA and lncRNA profile detected by transcriptomic high-throughput sequencing. a. Circos plot of genome coverage showed the detected reads were distributed in all chromosomes, similarly in each sample. S1, S2, S3, triplicate sample of the sham-operated group; B1, B2, B3, triplicate sample of bone cancer pain group. b. Heatmap of gene expression correlation between each sample. Pearson’s correlation coefficient within the same biological group was high, indicating intra-group consistency, and the coefficient in different groups was low, indicating inter-group variance. c. Statistic description of detected transcripts overall and differently expressed, respectively. d. Hierarchical cluster analysis of differently expressed mRNA (DEmRNA). The color scale indicates log10FPKM and intensity increases from green to red, which indicates down- and up-regulation, respectively. e. Hierarchical cluster analysis of differently expressed lncRNA (DElncRNA). f. volcano plot of DEmRNA, in which vertical lines correspond to 2-fold changes in upregulation or downregulation; horizontal line represents q = 0.05 (p value adjusted by FDR); red points refer to up-regulated mRNAs and blue points refer to down-regulated mRNAs. g. Volcano plot of DElncRNAs

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