Fig. 2

Tumor inoculation caused significant changes in mRNA and lncRNA profile detected by transcriptomic high-throughput sequencing. a. Circos plot of genome coverage showed the detected reads were distributed in all chromosomes, similarly in each sample. S1, S2, S3, triplicate sample of the sham-operated group; B1, B2, B3, triplicate sample of bone cancer pain group. b. Heatmap of gene expression correlation between each sample. Pearson’s correlation coefficient within the same biological group was high, indicating intra-group consistency, and the coefficient in different groups was low, indicating inter-group variance. c. Statistic description of detected transcripts overall and differently expressed, respectively. d. Hierarchical cluster analysis of differently expressed mRNA (DEmRNA). The color scale indicates log₁₀FPKM and intensity increases from green to red, which indicates down- and up-regulation, respectively. e. Hierarchical cluster analysis of differently expressed lncRNA (DElncRNA). f. volcano plot of DEmRNA, in which vertical lines correspond to 2-fold changes in upregulation or downregulation; horizontal line represents q = 0.05 (p value adjusted by FDR); red points refer to up-regulated mRNAs and blue points refer to down-regulated mRNAs. g. Volcano plot of DElncRNAs