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Fig. 3 | Molecular Brain

Fig. 3

From: Forebrain excitatory neuron-specific SENP2 knockout mouse displays hyperactivity, impaired learning and memory, and anxiolytic-like behavior

Fig. 3

SENP2 ablation impaired working memory, contextual fear learning and nest building activity. a-b Cognitive test of cKO mice. a Y maze test. cKO mice showed reduced percentage of accurate spontaneous alternations over total number of alternations among the three arms (Ctrl: 72.18 ± 1.84%, n = 18; cKO: 60.35 ± 4.22%, n = 10; p = 0.0062, Student’s t-test). b Contextual fear condition test. cKO mice showed low levels of freezing behaviors in training session (pre-shock: Ctrl: 3.25 ± 0.94%, n = 19; cKO: 2.66 ± 1.47%, n = 11, p > 0.9999; 1st shock: Ctrl: 7.83 ± 2.59%, n = 19; cKO: 0.89 ± 0.52%, n = 11, p > 0.9999; 2nd shock: Ctrl: 23.20 ± 4.06%, n = 19; cKO: 0.86 ± 0.48%, n = 11, p = 0.0009; 3rd shock: Ctrl: 39.05 ± 5.48%, n = 19; cKO: 1.11 ± 0.63%, n = 11, p < 0.0001; 4th shock: Ctrl: 48.83 ± 5.80%, n = 19; cKO:1.87 ± 1.11%, n = 11, p < 0.0001; 5th shock: Ctrl: 51.21 ± 4.96%, n = 19; cKO: 2.61 ± 1.01%, n = 11, p < 0.0001; two-way ANOVA analysis followed by Bonferroni’s post-hoc). In contextual fear retrieval session, cKO mice display very lowly freezing behaviors (2 min: Ctrl: 42.92 ± 4.97%, n = 19; cKO: 21.79 ± 4.21%, n = 11, p = 0.0481; 4 min: Ctrl: 50.61 ± 5.81%, n = 19; cKO: 14.55 ± 5.14%, n = 11, p < 0.0001; 6 min: Ctrl: 51.57 ± 5.35%, n = 19; cKO: 13.10 ± 4.79%, n = 11, p < 0.0001; 8 min: Ctrl: 52.49 ± 5.41%, n = 19; cKO: 14.05 ± 7.04%, n = 11, p < 0.0001; 10 min: Ctrl: 47.86 ± 5.02%, n = 19; cKO: 11.57 ± 4.60%, n = 11, p < 0.0001; two-way ANOVA analysis followed by Bonferroni’s post-hoc). c Nest building test. cKO mice display lower nest scores in the nest building test (Ctrl: 4.30 ± 0.26, n = 10; cKO: 1.44 ± 0.24, n = 9; p < 0.0001, Student’s t-test). d Acoustic startle response and prepulse inhibition in cKO mice. cKO mice display normal startle reflex in 120 dB acoustic stimulus compared with littermate controls (Ctrl: 475.71 ± 78.32, n = 8; cKO: 522.48 ± 58.08, n = 8; p = 0.6389, Student’s t-test) and showed similar PPI of the startle response than littermate control mice at the prepulse level of 70 dB, 74 dB, 78 dB (70 dB: Ctrl: 47.64 ± 5.23%, n = 8; cKO: 42.90 ± 3.01%, n = 8; p > 0.9999; 74 dB: Ctrl: Ctrl: 47.86 ± 6.92%, n = 8; cKO: 41.00 ± 3.84%, n = 8; p = 0.9895; 78 dB: Ctrl: 47.63 ± 6.44%, n = 8; cKO: 48.23 ± 2.21%, n = 8; p > 0.9999; two-way ANOVA analysis followed by Bonferroni’s post-hoc). All data presented as mean ± S.E.M.

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