Fig. 1
From: Pre- and post-synaptic roles for DCC in memory consolidation in the adult mouse hippocampus
Conditional deletion of DCC in CA1 pyramidal hippocampal neurons but not CA3 pyramidal hippocampal neurons impairs spatial memory performance in Barnes maze test. a-c R4ag11-Cre/DCCfl/fl (CA1 cKO) show no differences in latency to (a; Main effect of training: F4,104 = 2.54, p = 0.02; Interaction time by genotype: F4,104 = 1.10, p = 0.36) nor number of nose pokes in target hole (b; Main effect of training: F4,200 = 1.98, p = 0.044; Interaction time by genotype: F4,200 = 0.27, p = 0.89) during training phase, but spent significantly less time in the target hole quadrant compared to control littermates (black) (C; Interaction quadrant by genotype: F1,46 = 4.69, p = 0.03; Target quadrant, R4ag11-Cre/DCCf1/f1: 30.5 ± 4.7 s, Control: 45.0 ± 5.2 s; p = 0.007). Control, n = 13; CA1 cKO, n = 11. d-f Grik4-Cre/DCCfl/fl (CA3 cKO) did not show any significant differences in escape latency (d; Main effect of training: F5,70 = 14.77, p < 0.001; Interaction time by genotype: F5,70 = 0.77, p = 0.57) or nose pokes into target hole (e; Main effect of training: F4,56 = 7.67, p < 0.001; Interaction time by genotype: F4,56 = 0.96, p = 0.43) during training, and did not differ from control mice in time spent in target quadrant (f; t14 = 0.05, p = 0.96). Control, n = 10; CA3 cKO, n = 6. * denotes p < 0.05 using Bonferroni posthoc tests. Barnes maze tests for R4ag11-Cre/DCCfl/fl mice and Grik4-Cre/DCCfl/fl mice, both on a C57Bl/6 J background, were run by different investigators which may contribute to differences in baseline performance