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Fig. 8 | Molecular Brain

Fig. 8

From: Pre- and post-synaptic roles for DCC in memory consolidation in the adult mouse hippocampus

Fig. 8

Alterations in dendritic spine morphology in CA1 pyramidal neurons from R4ag11-Cre/DCCfl/fl mice. a Representative camera lucida reconstructions of CA1 pyramidal neuron apical dendrites from control (left) and R4ag11-Cre/DCCfl/fl mice. Scale bar = 1 μm. b Group data show no significant difference in average spine density between control and R4ag11-Cre/DCCfl/fl mice (left), but significant reductions in average spine length and spine head width (right). c Dendritic spine type classification reveals a significant increase in number of stubby-type spines (R4ag11-Cre/DCCfl/fl: 55.8 ± 4.6%, Control: 29.6 ± 0.9%; t5.4 = 5.59, p = 0.002) and significant decrease in the number of mushroom-type dendritic spines (R4ag11-Cre/DCCfl/fl: 40.6 ± 4.9%, Control: 68.2 ± 1.0%; t5.47 = 5.40, p = 0.002), with no detectable differences in thin-type spines (p = 0.41). d-i Group data (top) and representative Western blots from hippocampal homogenates of control and R4ag11-Cre/DCCfl/fl mice showing levels of SHANK1 (d), SHANK2 (e), SHANK3 (f), PSD-95 (g), p34-Arc (h), and phosphorylated S6 ribosomal protein (i). *: p < 0.05; **: p < 0.01. Data are presented as mean ± SEM

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