Fig. 6

Histograms of real-time RT-PCR results for the representative DAMPs-related genes. Histograms show the results of qRT-PCR at each time point (day 1, 3, 7, 14, 28 post-stroke). The results of cycle threshold values (Ct values) were calculated by the ΔΔCt method to obtain the fold differences. Five brains derived from each group (control and photothrombosis) were used for real-time PCR analysis at each time point. Data are expressed as fold change relative to sham-operated mice (control). The bars represent the mean ± SEM. The asterisks indicate a statistically significant difference compared with control (*p < 0.05, **p < 0.01 versus control, Dunnett’s multiple comparison test)