Fig. 7
The activation of CREB and GAP in the stroke brain. a The western blot analyses with anti-CREB and -GAP43 antibody. The brain lysates (30 μg per each lane) of whole ipsi-lateral cortices at day 1, 3, 7, 14, and 28 were blotted with anti-CREB and anti-GAP43 antibody, respectively. C (control). The lower histogram shows the semi-quantification of band intensities of phosphorylated-CREB and -GAP43 relative to the total CREB and GAP43 protein (C vs. day 14), respectively (*p < 0.05, C vs. day14, Dunnett’s multiple comparison test). The value of control was made 1 for normalization. b The immunohistochemistry with ant-phosphorylated CREB (pCREB) antibody on the brain section, at day 14 post-stroke. The top picture shows the cresyl-violet stained bran section at day 14. Scale bar = 100 μm (upper panels), 10 μm (lower panels). The lower histogram shows the semi-quantification of DAB-stained areas positive with anti- pCREB antibody in ischemic brain sections (Contra- vs. Ipsi-lateral cortex) (*p < 0.05, Contra- vs. Ipsi-lateral, Student’s t-test). The value of ipsilateral brain was made 100 for normalization. Scale bar = 50 μm (upper panels), 10 μm (lower panels). c The immunofluorescence pictures of peri-ischemic regions on the Ipsi- and Contra-lateral cortex at day 14, with anti-phosphorylated CREB (pCREB) and anti-NeuN antibody, respectively. NeuN; Neuron-Specific Nuclear Protein. DAPI; 4′,6-diamidino-2-phenylindole. Scale bar = 50 μm (lower panels)