Fig. 5
From: Chronic morphine regulates TRPM8 channels via MOR-PKCβ signaling
Activation of MOR by morphine does not change TRPM8 trafficking. a Western blot of surface biotinylated YFP-tagged TRPM8 in HEK cells. Top, surface biotinylation of untransfected cells, TRPM8-YFP or TRPM8-YFP + MOR transfected cells exposed to vehicle control or morphine (MS); bottom, western blot of plasma membrane Na+/K+ ATPase marker used as control. b Bar graph summarizing the biotinylation results illustrated in (a). Blots are representative, data are means ± SEM of the TRPM8 band intensity signal normalized to the Na+/K+ ATPase, from 4 independent experiments (unpaired t-test)