Fig. 6

Arginine prevents ischemic neuronal death by inhibiting inflammation response via suppression of HIF-1α/LDHA pathway. a TTC test shows arginine is neuroprotective, and no significant difference in infarct volume among arginine group, FX11 group, LW6 group, FX11 + arginine group and LW6 + arginine group in MCAO rats (n = 6 in each group, *p < 0.05 versus vehicle, one-way ANOVA test). b, c Cell viability test (b) and LDH release (c) show that administration of arginine in OGD microglia attenuates co-cultured neuron death, and no significance among arginine group, FX11 group, LW6 group, FX11 + arginine group and LW6 + arginine group (n = 6 in each group, *p < 0.05 versus vehicle, one-way ANOVA test). d, e, f mNSS test (d), beam-walking test (e), and MST test (f) show that there is no significant difference of functional recovery among arginine group, FX11 group, LW6 group, FX11 + arginine group and LW6 + arginine group (n = 6 in each group, *p < 0.05 versus Vehicle, two-way ANOVA test)