Fig. 1

The reduction in cortical interneurons in the postnatal cortex after Pdk1 ablation. (a) Real-time PCR showed that the level of Pdk1 transcription was decreased in the Pdk1 cKO subpallium at E16.5 (Ctrl: N = 4, Pdk1 cKO: N = 4, P = 0.004, Student’s t-test, with alpha = 0.05). Gapdh was used as an internal control. (b) Western blot analysis revealed decreased levels of PDK1 in the Pdk1 cKO subpallium at E16.5 (N = 4). GAPDH was used as an internal control. (c-d’) Immunostaining for GFP showed that the total number of cortical interneurons in the Pdk1 cKO cortex was decreased at P6. (e) Statistical analysis of GFP⁺ interneurons (Ctrl: N = 3, Pdk1 cKO: N = 3, P = 0.0013, Student’s t-test, with alpha = 0.05). (f-g’) In situ hybridization of SST showed a reduction in SST⁺ interneurons in the Pdk1 cKO cortex at P6. (h) Statistical analysis of SST⁺ interneurons (Ctrl: N = 3, Pdk1 cKO: N = 3, P = 0.023, Student’s t-test, with alpha = 0.05). (i-j’) In situ hybridization of PV at P15. (k) Statistical analysis of PV⁺ interneurons (Ctrl: N = 4, Pdk1 cKO: N = 4, P = 0.0013, Student’s t-test, with alpha = 0.05). (l-m’) Immunostaining for PROX1 revealed that the number of CGE-derived cortical interneurons in the Pdk1 cKO cortex was decreased at P6. (n) Statistical analysis of PROX1⁺ interneurons (Ctrl: N = 3, Pdk1 cKO: N = 3, P = 0.0013, Student’s t-test, with alpha = 0.05). The data are presented as the mean ± SEM. Scale bar, 100 μm