Fig. 5

The loss of Pdk1 resulted in increased cell apoptosis. (a-b′) Immunofluorescence showing that the number of Caspase-3⁺ cells was no differences in the subpallium of Pdk1 cKO and control mice at E12.5. (c-d’) The number of Caspase-3⁺ cells was significantly increased in the Pdk1 cKO subpallium compared with control at E14.5. (e-f′) Increased number of Caspase-3⁺ cells in the Pdk1 cKO subpallium compared to control at E16.5. (g-h′) Immunofluorescence for Caspase-3 showed that the number of Caspase-3⁺ cells was increased in the cortex of Pdk1 cKO mice compared with control mice at P6. (i) Statistical analysis of Caspase-3⁺ cells (E12.5 Pdk1 cKO: N = 3; Ctrl: N = 3; P = 0.9231; E14.5 Pdk1 cKO: N = 3; Ctrl: N = 3; P = 0.0048; E16.5 Pdk1 cKO: N = 3; Ctrl: N = 3; P = 0.0114; P6 Pdk1 cKO: N = 3; Ctrl: N = 3; P = 0.00004, Multiple t-test, with alpha = 0.05/4 = 0.0125). The data are presented as the mean ± SEM. Scale bar, 100 μm