Fig. 6
From: PDK1 regulates the survival of the developing cortical interneurons
Decreased activity of AKT-GSK3β signaling pathway after Pdk1 deletion. Western blotting analysis of the AKT-GSK3β signaling pathway in the subpallium at E16.5. (a) The relative expression levels of total AKT were no differences between Pdk1 cKO and control mice. (b) Statistical analysis of total AKT protein expression levels (Pdk1 cKO: N = 4; Ctrl: N = 4; P = 0.1186, Student’s t-test, with alpha = 0.05). (c) The relative expression levels of p-AKTThr308 was significantly reduced. (d) Statistical analysis of p-AKTThr308 protein expression levels (Pdk1 cKO: N = 5; Ctrl: N = 3; P = 0.0027, Student’s t-test, with alpha = 0.05). (e) While p-AKTSer473 was obviously increased in the Pdk1 cKO subpallium compare to control. (f) Statistical analysis of p-AKTSer473 protein expression levels (Pdk1 cKO: N = 4; Ctrl: N = 4; P = 0.0153, Student’s t-test, with alpha = 0.05). (g) There were no significant differences in the relative expression levels of total GSK3β between Pdk1 cKO and control mice. (h) Statistical analysis of total GSK3β protein expression levels (Pdk1 cKO: N = 4; Ctrl: N = 4; P = 0.5900, Student’s t-test, with alpha = 0.05). (i) The relative expression levels of p-GSK3βSer9 was significantly decreased in the Pdk1 cKO subpallium compared to control. (j) Statistical analysis of p-GSK3βSer9 protein expression levels (Pdk1 cKO: N = 4; Ctrl: N = 4; P = 0.0273, Student’s t-test, with alpha = 0.05). (k) There were no significant differences in the relative expression levels of PTEN between Pdk1 cKO and control mice. (l) Statistical analysis of PTEN protein expression levels (Pdk1 cKO: N = 4; Ctrl: N = 4; P = 0.8366, Student’s t-test, with alpha = 0.05). GAPDH was used as an internal control