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Fig. 2 | Molecular Brain

Fig. 2

From: Expression of serotonin 1A and 2A receptors in molecular- and projection-defined neurons of the mouse insular cortex

Fig. 2

Evidence of synaptic contacts of 5-HT1A+ insular neurons in the amygdala and lateral hypothalamus. a. Experimental design to detect synaptic terminals of 5-HT1A-expressing (5-HT1A+) insula neurons through cre-dependent expression of mCherry and synaptophysin-eYFP in the anterior or posterior insula of Htr1a-Cre mice. b. (Left) Confocal image of the cre-dependent viral vector injection site in a coronal section of the anterior (DI: dysgranular insula, AID: agranular insula dorsal part, AIV: agranular insula ventral part) and the posterior insula (GI: granular insula, AIP: agranular insula posterior part). Note mCherry expression in the soma of 5-HT1A+ neurons. (Right) Imaging locations of synaptophysin-eYFP in the basolateral and central amygdala (BLA and CeA) as well as the rostral and caudal part of the lateral hypothalamus (rLH and cLH). Distances are in the anteroposterior axis from Bregma. c-d. Confocal images of the expression of synaptophysin-eYFP in BLA, CeA, rLH and cLH, originating from 5-HT1A+ neurons of the anterior (c) and posterior (d) insula

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Molecular Brain

ISSN: 1756-6606