Skip to main content
Fig. 3 | Molecular Brain

Fig. 3

From: Expression of serotonin 1A and 2A receptors in molecular- and projection-defined neurons of the mouse insular cortex

Fig. 3

Expression of 5-HT1A in insula neurons projecting to subnuclei of the amygdala. a. Experimental timeline combining retrograde fluorescent tracers (CTB) injections and 5-HT1A immunostaining. b. Injection site of retrograde fluorescent tracers (CTB-555 and CTB-647) in the central and basolateral amygdala (CeA and BLA) within a horizontal brain slice (4.56 mm ventral to Bregma). c. Immunofluorescent image showing CTB-555 and CTB-647 positive neurons in the posterior insula. d-e. Representative fluorescent images of CTB-labelled neurons expressing 5-HT1A in the insula. Yellow arrows indicate 5-HT1A expression. f. Percentage of 5-HT1A expression in neurons projecting to the BLA and CeA in the anterior (ICa) and posterior insula (ICp). g. The proportion of 5-HT1A+ neurons is not different between IC-BLA and IC-CeA populations (unpaired t-test, p > 0.05). Total IC-BLA neurons: ICa + ICp = 417 + 620 = 1037; Total IC-CeA neurons: ICa + ICp = 455 + 647 = 1102; n = 7 mice, 2 locations per mouse

Back to article page

Molecular Brain

ISSN: 1756-6606