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Fig. 4 | Molecular Brain

Fig. 4

From: Expression of serotonin 1A and 2A receptors in molecular- and projection-defined neurons of the mouse insular cortex

Fig. 4

Expression of 5-HT1A in insular neurons projecting to the lateral hypothalamus. a. Experimental design combining retrograde fluorescent tracers (CTB) and serotonin 1A receptor (5-HT1A) antibody staining. b. Injection site of retrograde fluorescent tracers (CTB-555 and CTB-647) in the rostral and caudal parts of lateral hypothalamus (rLH and cLH, respectively) within a horizontal brain slice (4.56 mm ventral to Bregma). c. Percentage of 5-HT1A expression in neurons projecting to cLH and rLH from the anterior (ICa) and posterior (ICp) insula. d. The proportion of 5-HT1A+ neurons are not different between IC-rLH and IC-cLH populations (unpaired t-test, p > 0.05). Total IC-rLH neurons: ICa + ICp = 298 + 313 = 611; Total IC-CeA neurons: ICa + ICp = 422 + 244 = 666; n = 5 mice, 2 locations per mouse

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Molecular Brain

ISSN: 1756-6606