Fig. 3
From: Axl receptor tyrosine kinase is a regulator of apolipoprotein E
AZ7235-mediated apoE and ABCA1 induction is LXR-independent. U2-OS LXR-Gal4 chimeric Luciferase reporter assays were used to demonstrate that AZ7235 (1.5 nM – 30 μM) have no direct LXRα (a) or LXRβ (b) agonist activity, unlike the direct LXR agonist T091317, after 40 h of treatment. (c) Vehicle control, DMSO, 1 μM T0901317, 3 μM AZ7235 were added to CCF-STTG1 cells transfected with a mixture of LXR-responsive Firefly luciferase construct and constitutively expressing Renilla luciferase construct as an internal control. Luciferase activities were measured after 24 h of treatment. Error bars represent standard deviation from technical triplicates. *** P < 0.0001 by ANOVA post-hoc analysis. (d-e) LXR-knockout (LXRα−/β-) and LXRα-expressing (LXRα+/β-) MEF cells were treated with DMSO or drugs for 48 h. Apoe and Abca1 mRNA levels were measured by qRT-PCR. Graph represents fold-change over respective DMSO control (dash line) +/− 95% CI from 5 experiments