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Table 3 Analysis of Pan, A1, and A2 astrocyte signature gene expression by RNA-seq comparing prion-infected PLX5622-treated mice (microglia ablated) to similarly infected untreated mice (microglia present)

From: RNA-seq and network analysis reveal unique glial gene expression signatures during prion infection

Gene Description 80 dpia 100 dpi Terminalc
FCb P value FC P value FC P value
Pan
Aspg Asparaginase 1.8 3.0 × 10− 3 2.6 1.2 × 10− 6 1.5 1.9 × 10− 5
Cd44 CD44 antigen 1.6 5.9 × 10− 2 4.2 6.7 × 10− 6 2.0 3.2 × 10− 5
Gfap Glial fibrillary acidic protein 4.2 6.8 × 10− 4 4.5 1.7 × 10− 7 1.7 4.1 × 10− 6
Gfap Splice variant id MSTRG.4106 1.9 2.9 × 10− 2 3.2 1.5 × 10− 6 1.9 1.1 × 10− 4
Gfap Splice variant id MSTRG.4107 1.1 4.7 × 10− 1 1.8 4.8 × 10− 3 1.5 3.8 × 10− 3
Hspb1 Heat shock protein 1 1.6 1.2 × 10− 1 2.2 4.8 × 10− 2 2.1 2.7 × 10− 2
Lcn2 Lipocalin 2 1.5 1.4 × 10− 1 2.1 8.9 × 10− 3 2.1 3.9 × 10− 3
Osmr Oncostatin M receptor 1.8 3.0 × 10− 2 4.2 1.1 × 10− 5 2.1 3.6 × 10− 4
Serpina3n/3f/3 m Serine (or cysteine) peptidase inhibitor, clade A, member
3N /3F/ 3M
3.5 4.1 × 10− 4 5.0 1.1 × 10− 5 2.0 1.1 × 10− 4
Timp1 Tissue inhibitor of metalloproteinase 1 1.5 1.3 × 10− 1 2.2 1.6 × 10− 5 1.8 1.3 × 10− 4
Vim Vimentin 2.6 9.8 × 10− 3 5.7 1.2 × 10− 4 3.8 1.1 × 10− 6
A1
A2m Alpha-2-macroglobulin 1.4 8.5 × 10− 2 3.4 6.3 × 10− 5 1.9 3.3 × 10− 6
C3 Complement component 3 1.1 2.8 × 10− 1 2.4 6.6 × 10− 3 1.7 3.5 × 10− 5
C4b Complement component 4B (Chido blood group) 2.9 1.6 × 10− 4 3.0 1.2 × 10− 6 1.5 4.7 × 10− 6
Endou Endonuclease, polyU-specific 1.3 2.8 × 10− 2 1.5 4.3 × 10− 5 1.2 2.1 × 10− 2
Fbln5 Fibulin 5 1.1 2.6 × 10− 2 1.2 4.0 × 10− 2 1.9 4.7 × 10− 4
Fkbp5 FK506 binding protein 5 0.7 6.4 × 10− 2 1.0 8.3 × 10− 1 1.6 6.5 × 10− 2
Ggta1 Glycoprotein galactosyltransferase alpha 1, 3 1.5 3.1 × 10− 2 2.4 3.2 × 10− 5 1.4 1.7 × 10− 1
Serping1 Serine (or cysteine) peptidase inhibitor, clade G, member 1 1.6 7.8 × 10− 2 1.6 1.9 × 10− 2 2.0 1.6 × 10− 5
Slc22a4 Solute carrier family 22 (organic cation transporter), member 4 1.3 3.3 × 10− 3 1.5 7.3 × 10− 6 1.5 3.1 × 10− 4
Slc39a14 Solute carrier family 39 (zinc transporter), member 14 0.9 7.2 × 10− 1 1.5 5.0 × 10− 2 1.2 1.8 × 10− 1
Sorbs1 Sorbin and SH3 domain containing 1 1.0 9.7 × 10− 1 1.5 4.0 × 10− 3 1.3 1.1 × 10− 1
Sulf2 Sulfatase 2 1.0 8.6 × 10− 1 1.4 6.3 × 10− 3 1.7 7.2 × 10− 7
Tspan4 Tetraspanin 4 1.2 4.6 × 10− 1 1.3 1.6 × 10− 2 1.5 5.1 × 10− 2
A2
Anxa2 Annexin A2 1.3 2.8 × 10− 1 1.6 4.9 × 10− 3 1.6 1.4 × 10− 4
Cd109 CD109 antigen 1.2 2.1 × 10− 1 2.6 3.8 × 10− 4 2.7 4.3 × 10− 8
Emp1 Epithelial membrane protein 1 1.2 9.1 × 10− 2 2.2 2.6 × 10− 4 1.5 7.4 × 10− 2
Flnc Filamin C, gamma 1.5 1.7 × 10− 2 2.9 1.0 × 10− 5 2.1 1.7 × 10− 7
Gadd45b Growth arrest and DNA-damage-inducible 45 beta 1.2 5.3 × 10− 1 1.5 6.7 × 10− 4 1.4 5.8 × 10− 2
Hmox1 Heme oxygenase 1 1.5 6.8 × 10− 3 1.7 6.2 × 10− 4 1.2 2.0 × 10− 2
Lgals3 Lectin, galactose binding, soluble 3 1.3 5.4 × 10− 2 1.6 1.3 × 10− 3 1.5 7.8 × 10− 2
Lif Leukemia inhibitory factor 1.1 5.8 × 10− 2 1.7 6.3 × 10− 5 1.3 8.4 × 10− 5
Ptx3 Pentraxin related gene 1.2 1.2 × 10−1 2.0 8.2 × 10− 4 1.5 8.4 × 10− 5
S100a10 S100 calcium binding protein A10 (calpactin) 1.8 1.5 × 10−1 2.3 9.7 × 10− 3 1.5 9.3 × 10− 2
Sbno2 Strawberry notch homolog 2 1.3 1.3 × 10− 2 2.0 9.5 × 10− 6 1.4 2.5 × 10− 6
S1pr3 Sphingosine-1-phosphate receptor 3 1.5 3.9 × 10−1 4.1 3.0 × 10− 6 1.0 9.8 × 10− 1
Tgm1 Transglutaminase 1, K polypeptide 1.5 6.8 × 10− 3 3.4 4.7 × 10− 5 1.6 3.0 × 10− 4
Tm4sf1 Transmembrane 4 superfamily member 1 1.1 3.2 × 10−1 1.7 1.8 × 10− 3 1.7 2.1 × 10− 5
Tnfrsf12a Tumor necrosis factor receptor superfamily, member 12a 1.3 2.3 × 10−1 2.1 5.3 × 10−3 1.8 3.1 × 10− 3
  1. Bolded values denote genes increased ≥2.0-fold with p values ≤0.05 (5.0 × 10− 2) in RML-infected mice
  2. Values in italics are increased between 1.5-fold and 1.9-fold with p values ≤0.05 (5.0 × 10− 2) in RML-infected mice
  3. adpi days post inoculation
  4. bFC fold change
  5. c Terminal comparison is RML-infected mice at ~ 157 dpi relative to PLX5622-treated RML-infected mice at ~ 127 dpi