Fig. 1
Similar decrease in the number of dopaminergic neurons in SNpc of two alpha-synuclein knockout lines. a) The number of TH-positive neurons in the SNpc of 4-month old wild type (WT), B6(Cg)- Sncatm1.2Vlb/J alpha-synuclein null (SncaVlb) and B6(Cg)-Sncatm1Rosl/J alpha-synuclein null (SncaRosl) mice. Bar charts show mean ± SEM of numbers of stereologically counted neurons in SNpc of 8 animals for each genotype. Statistical analysis using one-way ANOVA with Dunnett’s multiple comparisons test revealed significant difference between WT and each of null mutant mouse groups (F (2, 45) = 4.854; p = 0.0123; **p = 0.0094; * p = 0.0451), whereas the difference between two null mutant mouse groups was not significant (p = 0.5329). b) Representative images of brain sections through SNpc region immunostained with antibody against TH