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Fig. 1 | Molecular Brain

Fig. 1

From: Foetal neural progenitors contribute to postnatal circuits formation ex vivo: an electrophysiological investigation

Fig. 1

Extra- and intra-cellular recordings reveal heterogeneity of the electrophysiological phenotype, across culture conditions. a Representative mixed cultures (phase contrast and confocal microscopy, respectively left and right top images, scale bar = 200 μm); bottom images display β tubulin III+ neurons in red, eGFP+ neurons in green and cells nuclei in blue surrounding the MEA microelectrodes (scale bars = 100 and 50 μm). Sample raw extracellular electrical potentials detected at single sites of three distinct MEAs, representative of our experimental conditions (b). Results from all the experiments, quantified in (c-f), suggesting that under both control conditions and disinhibition, HCC and hippocampal + fNPC mixed cultures, but not fNPC control cultures, detected spiking activity from the largest majority of MEA microelectrodes (not shown), with high rate of occurrence (c, d) and AP synchronization (e, f). **P < 0.01, ***P < 0.001, one-way ANOVA. When investigated by single-cell recordings, passive and active electrical cells’ properties reveal a distinct phenotype of fNPC in mixed cultures: input resistance (g) and capacitance (h). Note the inset explaining the legend. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Kruskal-Wallis. Pair recordings (i-j) were used to confirm the existence of synaptic connectivity between HCC and fNPC (10 cases out of 20) as well as the fraction of reciprocal connectivity (5 cases out of 20, see sample traces of panel i)

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