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Fig. 4 | Molecular Brain

Fig. 4

From: Differentiation and localization of interneurons in the developing spinal cord depends on DOT1L expression

Fig. 4

Dot1l-cKO decreases LHX2-dI1 interneurons and derails migration of the LHX9-dI1 interneurons at E11.5. (a, b) Representative immunostainings for LHX2 (red) and DAPI (gray) in (a) or LHX9 (red) and DAPI (gray) in (b) on spinal cord hemi-sections of control and mutant littermates at E11.5. Hemicord profiles highlighted by dotted white line. Scale bars: 100 μm. (c, d) Density plots from LHX2 (c) and LHX9 (d). Density plot projections were analyzed by multivariate analysis for Hotelling’s two-sample square test; *** p < 0.001, stars are reported on the Y axis (dorsoventral, DV) or X axis (mediolateral, ML) according to values on the individual axes. (e, f) Quantitative analyses of immunostainings for LHX2 (e) and LHX9 (f). Quantifications represented with mean ± SEM. P-values were calculated with unpaired, one-tailed (e) or two-tailed (f) Student’s t-test, * p < 0.05. Per staining, 4 hemi-sections were counted for each n (n = 3)

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