Fig. 6

Dot1l-cKO impairs the dI2 migration path. (a, b) Representative immunostainings for dI2 and V1 interneurons at E11.5 (a) and E12.5 (b). In (a), dorsal FOXD3 (red) labels dI2 interneurons and ventral FOXD3 marks V1, while DAPI (gray) stains all the nuclei. In (b), dI2 are dorsally marked by FOXD3 (red) and ventrally by the costaining of FOXD3 and BRN3A (green). V1 in (b) are ventral cells labeled uniquely by FOXD3. Hemicord profiles highlighted by dotted white line. Scale bars: 100 μm. (c, d) Density plots for dI2 interneurons at E11.5 (c) and E12.5 (d). Density plot projections were analyzed by multivariate analysis for Hotelling’s two-sample square test; *** p < 0.001. Stars are reported on the Y axis (dorsoventral, DV) or X axis (mediolateral, ML) according to values on the individual axes. (e, f) Quantitative analyses of immunostainings for dI2 at E11.5 (e) and E12.5 (f). Quantifications represented with mean ± SEM. P-values were calculated with unpaired, two-tailed Student’s t-test. Per staining, 4 hemi-sections were counted for each n (n = 3)