Fig. 7

DOT1L differentially controls dI5 early positioning and dI3 late distribution. (a) Representative immunostainings for dI3 and dI5 interneurons at E11.5. dI3 interneurons are labeled by TLX3 staining (red) and dI5 are labeled by both TLX3 and LMX1B (green), while DAPI (gray) stains all the nuclei. (b) Representative immunostainings for dI3 at E12.5. dI3 interneurons are labeled by ISL1/2 staining (red), while DAPI (gray) counterstains all the nuclei. (c, d) Density plots for dI5 interneurons at E11.5 (c) and dI3 interneurons at E12.5 (d). Density plot projections were analyzed by multivariate analysis for Hotelling’s two-sample square test; *** p < 0.001. Stars are reported on the Y axis (dorsoventral, DV) or X axis (mediolateral, ML) according to values on the individual axes. (e, f) Quantitative analyses of immunostainings for dI5 at E11.5 (e) and dI3 at E12.5 (f). (g, h) Representative immunostainings of LBX1 (red) and LMX1B (green) for excitatory interneurons (g) and PAX2 (red) in costaining with FOXP1 (green) for inhibitory interneurons (h) at E12.5. Hemicord profiles highlighted by dotted white line. Scale bars: 100 μm. Quantifications represented with mean ± SEM. P-values were calculated with unpaired, two-tailed Student’s t-test. Per staining, 4 hemi-sections were counted for each n (n = 3)