Fig. 8

DOT1L depletion at E12.5 causes distributional defects of V0 and reduces V1 migration. (a) Representative immunostainings for EVX1 (green) and DAPI (gray) on spinal cord hemi-sections of control and mutant littermates at E12.5. Hemicord profiles highlighted by dotted white line. Scale bars: 100 μm. (b) Density plots from EVX1-expressing cells. (c) Quantitative analysis of EVX1, represented with mean ± SEM. (d, e) Density plots for V1 interneurons from Fig. 6a (E11.5, ventral cells FOXD3-expressing) and 6b (E12.5, ventral cells FOXD3 single-labeled), respectively at E11.5 (d) and E12.5 (e). Density plot projections were analyzed by multivariate analysis for Hotelling’s two-sample square test; ** p < 0.005, *** p < 0.001, stars are reported on the Y axis (dorsoventral, DV) or X axis (mediolateral, ML) according to values on the individual axes. (f, g) Quantitative analyses of immunostainings for FOXD3-positive V1 at E11.5 (f) and E12.5 (g). P-values were calculated with unpaired, two-tailed Student’s t-test. 4 hemi-sections were counted for each n (n = 3)