Fig. 1
From: The DISC1 R264Q variant increases affinity for the dopamine D2 receptor and increases GSK3 activity
The R264Q DISC1 variant facilitates the DISC1-D2R complex formation. a. DISC1-D2R complex formation is significantly increased in HEK-293 T cells expressing HA-D2R/Flag-DISC1R264Q (R264Q) compared to HEK-293 T cells expressing HA-D2R/Flag-DISC1 (DISC1). Cell lysate was subjected to immunoprecipitation with anti-HA or IgG and immunoblotted with anti-Flag or anti-HA antibodies. b-c. Densitometric analysis of precipitated DISC1 or D2R. The intensity of DISC1 or D2R was quantified by densitometry (software: ImageLab, Bio-Rad). *p < 0.05. d. DISC1-D2R complex formation does not change in HEK-293 T cells expressing HA-D2R/Flag-DISC1A83V (A83V) compared to HEK-293 T cells expressing HA-D2R/Flag-DISC1 (WT). Cell lysate was subjected to immunoprecipitation with anti-HA or IgG and immunoblotted with anti-Flag or anti-HA antibodies. e-f. Densitometric analysis of precipitated DISC1 or D2R. The intensity of DISC1 or D2R was quantified by densitometry (software: ImageLab, Bio-Rad). Data was shown as mean ± SEM, and was analyzed by t-test. n = 3