Fig. 2From: The DISC1 R264Q variant increases affinity for the dopamine D2 receptor and increases GSK3 activityThe R264Q DISC1 variant enhances the binding affinity between DISC1 and D2R. Surface Plasmon Resonance (SPR) analysis of the concentration-dependent binding of GST-DISC1NT (a) or GST-DISC1NT-R264Q (b) with immobilized TAT-D2pep[K211-T225] peptide. Binding affinity was measured using a range of concentrations from 0 nM to 4000 nM. The duration of the association and dissociation phases was set to 180 s, 300 s, respectively. Flow rate was set to 50 uL/min to reduce the effect of mass transport limitations. Regeneration of the sensor chip was achieved by adding 10 mM NaOH, 1 M NaCl. Equilibrium analysis was conducted for GST-DISC1NT based on the binding affinity of the interactionsBack to article page