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Fig. 2 | Molecular Brain

Fig. 2

From: The DISC1 R264Q variant increases affinity for the dopamine D2 receptor and increases GSK3 activity

Fig. 2

The R264Q DISC1 variant enhances the binding affinity between DISC1 and D2R. Surface Plasmon Resonance (SPR) analysis of the concentration-dependent binding of GST-DISC1NT (a) or GST-DISC1NT-R264Q (b) with immobilized TAT-D2pep[K211-T225] peptide. Binding affinity was measured using a range of concentrations from 0 nM to 4000 nM. The duration of the association and dissociation phases was set to 180 s, 300 s, respectively. Flow rate was set to 50 uL/min to reduce the effect of mass transport limitations. Regeneration of the sensor chip was achieved by adding 10 mM NaOH, 1 M NaCl. Equilibrium analysis was conducted for GST-DISC1NT based on the binding affinity of the interactions

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