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Fig. 3 | Molecular Brain

Fig. 3

From: The DISC1 R264Q variant increases affinity for the dopamine D2 receptor and increases GSK3 activity

Fig. 3

The DISC1R264Q variant decreases GSK3 α/β Ser21/9 phosphorylation. a. Activation of D2R reduces GSK-3α/β (Ser21/9) phosphorylation in HEK-293 T cells expressing D2R and DISC1, D2R and DISC1-R264Q, but not in HEK-293 cells expressing D2R with pcDNA3, the mammalian expression vector in which DISC1 is sub-cloned. Western blot analysis of phosphorylated GSK-3α/β levels in extract prepared from HEK 293 T cells transfected with D2R with pcDNA3 or D2R with DISC1 or D2R and DISC1-R264Q in the presence or absence of quinpirole (10 µM, 30 min). GSK-3α/β was used as a loading control. b-c. Densitometric analysis of phosphorylated GSK-3α/β (Ser21/9). The intensity of phospho-GSK-3α/β (Ser21/9) was quantified by densitometry (software: ImageLab, Bio-Rad). Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test. (*p < 0.05, ***p < 0.001, compared to D2R + DISC1-WT group, #p < 0.05 as compared to D2R + DISC1-R264Q, n = 6). Data was shown as mean ± SEM

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