Fig. 5
From: Drp1 is widely, yet heterogeneously, distributed in the mouse central nervous system
Comparison of Drp1 mRNA and protein expression. a. Comparison of Drp1 mRNA and protein expression in the brain. Based on the Allen Mouse Brain Atlas, the Drp1 signal was visualized in four different brain coronal sections. The left part shows the Drp1 mRNA expression, and the right part shows the Drp1 protein expression. b. Comparison of Drp1 mRNA and protein expression in the spinal cord. Drp1 mRNA was highly expressed in layer I; in the deep layers (VII, VIII, IX, X), Drp1 protein expression was slightly higher than in the superficial layers (I, II, III). c. Statistical analysis of the Drp1 mRNA and protein expression comparison in the brain. Drp1 expression was determined based on the labeling intensity, which was classified as no signal (−), low signal (+), moderate signal (++), high signal (+++), and strong signal (++++). The expression levels of Drp1 mRNA and protein were statistically analyzed based on 148 mice brain nuclei. The results showed that the Drp1 mRNA and protein expression levels were different in the mouse brain. In the brain, high expression of Drp1 mRNA (“+ + +” accounting for 53%) but low expression of Drp1 protein was mostly observed (“+” accounting for 59%). D. Statistical analysis of Drp1 mRNA and protein expression comparison in the spinal cord. In the spinal cord, high expression of Drp1 mRNA (“+ + +” accounting for 90%) but low expression of Drp1 protein was mostly observed (“+” accounting for 60%)