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Fig. 1 | Molecular Brain

Fig. 1

From: Receptor protein tyrosine phosphatase delta is not essential for synapse maintenance or transmission at hippocampal synapses

Fig. 1

Generation of PTPδ-cKO mice. a cKO strategy for the PTPδ mouse line. Upper: Targeting of exon 12 of the PTPδ gene. Bottom left: Primer locations for WT and floxed alleles (arrows). Bottom right: PCR genotyping of WT and PTPδfl/fl mice. b, Quantitative RT-PCR analysis of neuronal mRNA. Relative levels of PTPσ, PTPδ, and LAR mRNAs were measured in cultured cortical neurons infected with lentiviruses expressing Cre-recombinase. Data are means ± SEMs (n = 3–4 independent experiments; *p < 0.05; Mann Whitney U-test). c, d, Representative immunoblot image (c) and quantitative analyses (d) of the level of PTPδ protein in cultured cortical neurons infected with lentiviruses expressing Cre-recombinase. Data are means ± SEMs (n = 6 independent experiments; **p < 0.01; Mann Whitney U-test). e, Representative immunoblot analysis of the level of PTPδ protein in brain homogenates from 8-week old control and PTPδ-cKO mice. Levels of PTPδ protein were measured in PTPδfl/fl mice (Ctrl) or PTPδfl/fl mice crossed with Nestin-Cre mice (Nestin-PTPδ). Arrows indicate band(s) that show immunoreactivity to PTPδ-specific (JK179) antibodies. f, An image illustrating the body size of littermate control (Ctrl) and Nestin-PTPδ mice at 2 months of age. Nestin-PTPδ mice were significantly smaller than age- and sex-matched Ctrl mice

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