Skip to main content
Fig. 2 | Molecular Brain

Fig. 2

From: Receptor protein tyrosine phosphatase delta is not essential for synapse maintenance or transmission at hippocampal synapses

Fig. 2

Loss of PTPδ does not affect synapse development or synaptic transmission in cultured hippocampal neurons. a, PTPδ cKO in cultured hippocampal neurons does not affect excitatory or inhibitory synapse density. Double-immunofluorescence analysis of MAP2 (blue) and the indicated synaptic markers (red) in mature cultured neurons (DIV14) derived from PTPδf/f mice, infected with lentiviruses expressing ΔCre or Cre at DIV3. Synaptic markers assayed include the excitatory synaptic markers, surface GluA1 (sGluA1), total GluA1, Shank and VGLUT1, and the inhibitory synaptic markers, surface GABAAγ2 (sGAγ2), total GABAAγ2, and gephyrin (Gphn). Scale bar: 10 μm. b, Quantification of images in (a), measuring the density of the indicated synaptic markers. Data are means ± SEMs (n denotes the number of analyzed neurons; ∆Cre/sGluA1, n = 14; Cre/sGluA1, n = 14; ∆Cre/GluA1, n = 14; Cre/GluA1, n = 15; ∆Cre/Shank, n = 21; Cre/Shank, n = 21; ∆Cre/VGLUT1, n = 16; Cre/VGLUT1, n = 16; ∆Cre/sGABAARγ2, n = 16; Cre/sGABAARγ2, n = 17; ∆Cre/GABAARγ2, n = 10; Cre/GABAARγ2, n = 9; ∆Cre/Gephyrin, n = 16; and Cre/Gephyrin, n = 16, n = 16; Mann Whitney U-test). c, d, Representative mEPSC traces (c) and quantification of frequencies, amplitudes and kinetics (d) of mEPSCs recorded from cultured hippocampal neurons derived from PTPδf/f mice infected with lentiviruses expressing ΔCre or Cre. Data are means ± SEMs (n denotes the number of analyzed neurons; ΔCre, n = 19; Cre, n = 13; unpaired t-test). e, f, Representative mIPSC traces (e) and quantification of frequencies, amplitudes and kinetics (f) of mIPSCs recorded from cultured hippocampal neurons derived from PTPδf/f mice infected with lentiviruses expressing ΔCre or Cre. Data are means ± SEMs (n denotes the number of analyzed neurons; ΔCre, n = 18; Cre, n = 16; unpaired t-test)

Back to article page