Fig. 9
From: Differential expression of Cdk5-phosphorylated CRMP2 following a spared nerve injury
CRMP2 is expressed and phosphorylated in the dorsal horn of the spinal cord. Micrographs of a 20 μm section of adult spinal cord from animals having received a spared nerve injury and immunostained with antibodies against (a) total CRMP2 or (c) CRMP2 p522 as indicated. IB4 was used to stain superficial laminae of the dorsal horn. CRMP2 and CRMP2 p522 immunoreactivity was found in spinal cord neurons and colocalized with IB4 (indicated by arrows). N = 4 animals; ipsi = ipsilateral (injured side) and contra = contralateral (non-injured side). Bar graphs showing the comparison between contralateral and ipsilateral side of a spared nerve injury for (b) total CRMP2 or (d) CRMP2 p522 signal in the dorsal horn of the spinal cord. Data is shown as mean ± SEM, *p < 0.05; Student’s t-test compared to the contralateral side