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Fig. 1 | Molecular Brain

Fig. 1

From: Intraventricular IL-17A administration activates microglia and alters their localization in the mouse embryo cerebral cortex

Fig. 1

Experimental design and measurement of Iba1+ cells and CD68+ microglia in E18.5 cortex. a Experimental design of IL-17A injection to the lateral ventricle and brain sampling. Immunostaining of Iba1 (b) and CD68 (c) in E18.5 mouse brain. d Examples of ROIs (300 × 300 μm) placed on cingulate (medial) and primary sensory (lateral) cortex. b Position of bins 1 to 6 (each 50 × 300 μm) in the ROI. Example of counting Iba1 cells (c) and CD68 microglia (d). In all images, left is lateral and right is the medial side of the brain. Green: Iba1, Red: CD68, Blue: DAPI, Scale bars = 100 μm. CC: corpus callosum, LV: lateral ventricle, SC: somatosensory cortex, SVZ: subventricular zone, VZ: ventricular zone

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Molecular Brain

ISSN: 1756-6606