Fig. 1
From: Selective inhibition of neuronal Cav3.3 T-type calcium channels by TAT-based channel peptide
Effect of TAT-C3P peptide on Cav3 channels. a Schematic representation of the membrane topology of Cav3 channels and protein sequence alignment showing the conserved proximal region of the carboxy terminus across all three Cav3 channel isoforms (Cav3.1, Cav3.2, and Cav3.3). Molecular modeling indicates that this region may adopt a helical conformation. b Representative T-type current traces recorded from cells expressing Cav3.3 channels in response to 300 ms depolarizing steps to values ranging from − 80 mV to + 20 mV from a holding potential of − 100 mV for control (black traces) and TAT-C3P-treated cells (red traces). c Corresponding mean peak current density-voltage (I/V) relationship. d Corresponding mean maximal macroscopic conductance (Gmax) values obtained from the fit of the I/V curves with a modified Boltzmann equation for control (white dots), and cells treated with either TAT-C3P (red dots) or the control TAT-C3D peptide (grey dots). e Mean normalized Gmax for cells expressing Cav3.1, Cav3.2, and Cav3.3 channels in response to TAT-C3P treatment