Skip to main content
Fig. 2 | Molecular Brain

Fig. 2

From: Neural circuit analysis using a novel intersectional split intein-mediated split-Cre recombinase system

Fig. 2

Design of luciferase assay. HEK293T cells were transfected with Cre recombinase, NCre-IntN or IntC-CCre alone, or together, with a LoxP-stop-LoxP-Luciferase reporter. a Schematic showing the experimental design for transfecting cDNA constructs in HEK293T cells. b The Cre activity is defined by the amount of luminescence detected in the luciferase assay. The mean difference for 4 comparisons against the shared control Cre recombinase are shown in the above Cumming estimation plot. The raw data is plotted on the upper axes. On the lower axes, mean differences are plotted as bootstrap sampling distributions. Each mean difference is depicted as a dot. Each 95% confidence interval is indicated by the ends of the vertical error bars. Importantly, we observed similar levels of Cre activity for both the native Cre recombinase and the reconstituted split-Cre recombinase. * p < 0.05, ** p < 0.01, *** p < 0.001

Back to article page